Evaluation of a leukocyte stabilization reagent for use in the cytomegalovirus pp65 antigenemia assay.
نویسندگان
چکیده
New erythrocyte lysis and leukocyte stabilization reagents (Streck Laboratories, Inc.) were tested in the cytomegalovirus pp65 antigenemia assay, to determine if whole-blood processing time could be delayed to 24 h postdraw. The combination of these reagents gave results comparable to those for patient samples processed immediately after blood draw.
منابع مشابه
Leukocyte concentration in the performance of the pp65 antigenemia assay.
One of the more controversial and debatable issues presently confronting clinical virologists and infectious disease specialists is the application of the cytomegalovirus antigenemia (CMV-Ag) assay (pp65 antigenemia assay) as a marker and predictor of CMV disease. For example, recent reports have shown the CMV-Ag assay to be more accurate than conventional DNA PCR or the more recent nucleic aci...
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In a multicenter study, 113 blood samples from 19 organ transplant patients were analyzed for cytomegalovirus by the pp65 antigenemia assay and a quantitative DNA hybridization assay. Overall, there was 84% agreement among the results obtained by the two tests. Fifteen of 16 episodes of active infection were detected by both assays. One episode was missed by the pp65 assay, and one patient show...
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We compared the Digene Hybrid Capture CMV DNA Assay version 2.0, the pp65 antigenemia assay, traditional tube culture, and shell vial culture for the detection of cytomegalovirus (CMV) viremia in several patient populations at three centers. Of 561 blood specimens collected from 402 patients, complete clinical and laboratory data were available for 489. Using consensus definitions for true posi...
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BACKGROUND Quantitative real-time PCR assays, which are more rapid and practical than pp65 antigenemia determination, are progressively becoming the preferred method for monitoring Human Cytomegalovirus (HCMV) reactivation. However, the relationship between HCMV DNA and antigenemia levels is still under investigation. The aim of this study was to analyse the relationship between HCMV DNA and pp...
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The performance of a commercially available qualitative PCR test for plasma (AMPLICOR CMV Test; Roche Diagnostics) and a quantitative PCR test for plasma and leukocytes (COBAS AMPLICOR CMV MONITOR Test; Roche Diagnostics) was evaluated with samples from 50 blood or marrow allogeneic transplant recipients who received short courses of sequential ganciclovir therapy (2 weeks intravenously followe...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 36 11 شماره
صفحات -
تاریخ انتشار 1998